INTRODUCTION This section introduces the reader to the study and why the study was done. This should only be a few sentences long. For example: “…There are many reasons for identifying an unknown bacterium. The reasons range from…[explain 2-3 reasons why you may want to identify an unknown bacterium]. This study was done by applying all of the methods that have been learned in the microbiology laboratory class for the identification of an unknown bacterium…” RESULTS This is where the results are summarized. The method results should be in a table format Unknown Identity: Microscopic Morphology Characteristic Observation Gram Stain Other Staining (if any) Morphology Arrangement (if any) Macroscopic Morphology Characteristic Description Color/ Pigmentation Biochemical Tests Test Result Appearance/Description Methyl Red Voges-Proskauer Citrate Carbohydrate Fermentation Glucose Lactose Sucrose Enzymes Catalase Amylase Kligler Iron Agar Carbohydrates H2S Production Actions Record the color changes or the reactions that were observed. Record results appropriately, according to the test. Include a legend for abbreviations or any use of letter. DISCUSSION/CONCLUSION There should be 2 parts: This section interprets the meaning of the results. How did the test results lead to identification? When discussing the results, specify which organisms were ruled-out and which were ruled-in by each test result. This provides the logic and analysis for the identification of the unknown. Background information about the identified organism (environment preferred, pathogenicity, interesting cellular structures, an interesting fact, etc.). Example of a discussion: After several differential tests, it was concluded that unknown G was Escherichia coli. After performing the Gram stain to determine that the unknown was a Gram negative rod, the organism was grown on a tryptic soy agar slant for use in inoculating the rest of the biochemical tests. All of the biochemical tests worked well except for the indole test. It gave a false negative result at first. This was determined since it was inconsistent with the rest of the result. It was suggested that the test be repeated and it was repeated. The repeated test gave a positive result, consistent with the other data. Therefore, it was concluded that the unknown was Escherichia coli. THIS CAN BE ELABORATED MORE FOR EACH TEST. E. coli is in the Enterobacteriaceae family. It is typically found in the intestines of humans, as well as other mammals. It can cause disease in the right host. THE REST OF THIS INFORMATION SHOULD BE RESEARCHED FROM THE TEXTBOOK, INTERNET RELIABLE SOURCE OR OTHER MICROBIOLOGY RESOURCES. REFERENCES The minimum number of references is 3, which MUST include the lab manual OR textbook, and the CDC website. The other reference may NOT be Wikipedia or sites that end in .com or .net (typically not reliable). References should be listed in APA format (http://www.wooster.edu/psychology/apa-crib.html (Links to an external site.) and http://citationmachine.net (Links to an external site.) ) and in-text citations should be used where appropriate. Laboratory notes should be referenced as such: Bottoms, C., & Cain, D. (2006). Microbiology laboratory notes. 1 ed. Plano, TX: Collin County Community College.
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