Molecular Biology and Genetics


Assignment made up in 6 topics (10 marks each) which are consolidate activities on designated weeks noted WEEK 3: Hardy-Weinberg Equilibrium Explore the Hardy-Weinberg Equilibrium equations with The Amoeba Sisters! Learn why this equation can be useful, its five assumptions, and how to calculate genotype and allele frequencies with p and q values! This video does assume the viewer already knows vocabulary such as genotype, phenotype, and alleles from our previous videos … WEEK 4: WEEK 5: a DNA absorbs UV light very efficiently, with the nitrogenous bases in nucleotides showing maximum absorption at about 260 nm.  An absorbance at 260 nm in a 1 cm quartz cuvette of 1 is equivalent to approximately 50 µg/ml dsDNA, 33 µg/ml ssDNA, or 40 µg/ml for RNA. You can calculate the concentration of the DNA in your sample as follows: DNA concentration (µg/ml) = (A260) x (dilution factor) x (50) In contrast to nucleic acids, proteins have a maximum UV absorption of 280 nm (due mostly to the tryptophan residues). Thus the absorbance of a DNA sample at 280 nm gives an estimate of the protein contamination of the sample. The ratio of the A260/A280 is a measure of the purity of a DNA sample.  Pure DNA should have a ratio of approximately 1.8 and pure RNA 2.0.  A lower ratio indicates protein contamination. Absorption at 230 nm reflects contamination of the sample by substances such as carbohydrates, peptides, phenols or aromatic compounds. In the case of pure DNA samples, the ratio A260/A230 should be approximately 2.2. The relationship between concentration and absorbance is only proportional over a certain range of readings (A260 values greater than 0.1 and less than 1.0).  Absorbance readings outside this range cannot be used to calculate concentration and the dilution of the sample should be increased or decreased, as appropriate, to obtain a reading within this range. WEEK 6: Wellcome Library | History of genetics timeline History of genetics timeline. The library reopens on 7 October, along with the rest of Wellcome Collection week 8: (the files named mol biol translation and mol biol transcription as well as the links attached). * What is the ‘Central Dogma’? | Facts | The central dogma of molecular biology explains the flow of genetic information, from DNA to RNA, to make a functional product, a protein.; The central dogma suggests that DNA contains the information needed to make all of our proteins, and that RNA is a messenger that carries this information to the ribosomes.; The ribosomes serve as factories in the cell where the information is …

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